ddRADSeq of Poecilimon luschani: muscle - SRA

SRX20527431: ddRADSeq of Poecilimon luschani: muscle
1 ILLUMINA (Illumina HiSeq 2500) run: 3.1M spots, 438.3M bases, 237Mb downloads

Design: We used Qiagen DNeasy kits (Qiagen, Hilden, Germany) to extract and purify genomic DNA from the hind femur of each individual. We processed genomic DNA into one genomic library (100 individuals/library) using the double-digestion restriction-site associated DNA sequencing procedure (ddRAD-seq) described in Peterson et al. (2012) (PLOS One, 7: e37135). In brief, we digested DNA with the restriction enzymes MseI and EcoRI (New England Biolabs, Ipswich, MA, USA) and ligated Illumina adaptors including unique 11-bp barcodes to the digested fragments of each individual. We pooled ligation products and size-selected them between 350-450 bp with a Pippin Prep machine (Sage Science, Beverly, MA, USA). We amplified the fragments by PCR with 12 cycles using the iProofTM High-Fidelity DNA Polymerase (BIO-RAD, Hercules, CA, USA) and sequenced the library in two single-read 150-bp lanes on an Illumina HiSeq2500 platform at The Centre for Applied Genomics (SickKids, Toronto, ON, Canada).

Submitted by: Estacion Biologica de Donana (EBD-CSIC)

Study: RADseq for Poecilimon (Orthoptera: Tettigoniidae)

PRJNA976820 • SRP439870 • All experiments • All runs

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The goal of this project is to use next generation sequencing (NGS) data to resolve taxonomic uncertainties and infer the demographic processes underlying the diversification of bush crickets from the genus Poecilimon (Orthoptera: Tettigoniidae).

Sample:

SAMN35443022 • SRS17836720 • All experiments • All runs

Organism: Poecilimon luschani

Library:

Name: PLulGele06

Instrument: Illumina HiSeq 2500

Strategy: RAD-Seq

Source: GENOMIC

Selection: Restriction Digest

Layout: SINGLE

Runs: 1 run, 3.1M spots, 438.3M bases, 237Mb

Run	# of Spots	# of Bases	Size	Published
SRR24751250	3,130,461	438.3M	237Mb	2023-10-26

ID:: 27951140

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Sequence Read Archive

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